USP11 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
USP11
Enzyme
Human USP11
Synonym
UHX1, Ubiquitin carboxyl-terminal hydrolase 11, Deubiquitinating enzyme 11, Ubiquitin thioesterase 11, Ubiquitin-specific-processing protease 11
Substrate
Ubiquitin Rhodamine 110 Protein, 100 nM
Assay Properties
Reaction
Ubiquitin Rhodamine 110 = Ubiquitin + Rhodamine 110
Rhodamine 110 fluorescence, kinetic measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Mitoxantrone | 1765 |
| UCH-L1 Inhibitor | 39,590 |
| UCH-L3 Inhibitor | 16,630 |
Scientific Information
P51784 (https://www.uniprot.org/uniprotkb/P51784/entry)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Deubiquitination Assays (DUB assays)
Reference Compound IC50 for USP11
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