USP28 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
USP28
Enzyme
Human USP28 (Catalytic Domain)
Synonym
KIAA1515
Scientific Information
Q96RU2 (https://www.uniprot.org/uniprotkb/Q96RU2)
Assay Properties
Substrate
100 nM Ubiquitin Rhodamine 110 Protein
Reaction
Ubiquitin Rhodamine 110 = Ubiquitin + Rhodamine 110
Rhodamine 110 fluorescence, kinetic measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| AZ1 | 0.24 |
| UCH-L1 Inhibitor | 7.6 |
| UCH-L1 Inhibitor 2 | 33.8 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Deubiquitination Assays (DUB assays)
Reference Compound IC50 for USP28
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