PXR Nuclear Receptor Assay for compound screening in intact cells via luciferase reporter assay to quantify nuclear receptor activity.
Target Overview
Target
Pregnane X Receptor (PXR)
HGNC symbol
NR1I2
Synonyms
Steroid and xenobiotic sensing nuclear receptor (SXR)
Cell line
Human PXR Reporter Cell Line
Assay description:
– assay format: 96-well format
– construct: the PXR receptor contains the yeast GAL4 DNA binding sequence, which is functionally linked to a resident luciferase reporter gene (INDIGO biosciences)
– readout: receptor activity
Assay Properties
Further information
More information can be found on our website Nuclear Receptor Assay Services.
Human PXR Reporter cells were plated in 96-well plate and then stimulated with various concentrations of reference agonist Rifampicin for 24 hours. Luminescence signal was measured with an EnVision 2104 Multilabel Reader. PXR activation dose-response curve was plotted using GraphPad Prism program.
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