Surface Plasmon Resonance (SPR) technique for evaluating direct binding of small molecule inhibitors to BCL-2 and mutants F104L, D103Y, and G101V
Target Overview
Target
B-cell lymphoma-2
HGNC symbol
BCL2
Synonyms
BCL-2, PPP1R50, B-cell CLL/lymphoma 2, apoptosis regulator, BCL2 apoptosis regulator
Scientific information
P10415 (https://www.uniprot.org/uniprot/P10415)
Protein source
Produced at Reaction Biology
Screening location
Malvern, PA, USA
Further information
More information can be found on our website SPR Assay Services for Drug Discovery
Binding kinetics of ABT-737 to BCL-2 wildtype and mutants F104L, D103Y, and G101V
The data was collected on a Biacore 8K using single-cycle kinetics. The data was analyzed using a 1:1 kinetic binding model.
PDF