Surface Plasmon Resonance (SPR) technique for evaluating direct binding of BRD3-2 to molecular inhibitors
Target Overview
Target
Bromodomain-containing protein 3 domain 2 (aa 306-416)
HGNC symbol
BRD3
Synonyms
RING3-like protein
Uniprot page
Q15059 (https://www.uniprot.org/uniprot/Q15059)
Protein source
Reaction Biology Cat# RD-11-164
Screening location
Malvern, PA, USA
Further information
More information can be found on our website SPR Assay Services for Drug Discovery
Binding of three BET bromodomain inhibitors to BRD3-2
The data was collected on a Biacore 8K using multi-cycle kinetics. The data was analyzed using a 1:1 kinetic binding model or steady state affinity model to determine binding affinity (KD).
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