Surface Plasmon Resonance (SPR) technique for evaluating direct binding of HRAS to molecular inhibitors
Target Overview
Target
H-Ras
HGNC symbol
HRAS
Synonyms
HRAS, HRAS1
Protein
H-Ras (aa 2-169) with a C-terminal biotinylation expressed in E. coli
Scientific Information
P01112 (https://www.uniprot.org/uniprot/P01112)
Screening location
Malvern, PA, USA
Further information
More information can be found on our website SPR Assay Services for Drug Discovery
Binding of reference compound (BI-2852) to H-RAS
The data was collected on a Biacore 8K using multi-cycle kinetics. The data was analyzed using a 1:1 kinetic binding model to determine binding affinity (KD). Data shown here was collected for “as is” protein assumed to be in the GDP bound state.
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