ALK Cellular Phosphorylation Assay Service

The anaplastic lymphoma receptor tyrosine kinase ALK, which is highly expressed in the neonatal brain, belongs to the Insulin receptor superfamily. The kinase is involved in the development and maintenance of the central and peripheral nervous system. In most anaplastic large-cell non-Hodgkin’s lymphomas developed from activated T lymphocytes, a (2;5) chromosomal translocation is found, which causes a fusion of the nucleophosmin gene NPM to the catalytic domain of ALK (NPM-ALK). This gene fusion results in a constitutive ligand-independent kinase activation.

ALK

CD246, NBLST3

Karpas-299

Endogenous

The human T cell lymphoma cell line Karpas-299 carries a (2;5) chromosomal translocation, which results in the expression of an ALK variant fused to nucleophosmin (NPM-ALK). This fusion results in constitutive, ligandindependent receptor tyrosine autophosphorylation of ALK, which can be reduced by adding ALK specific inhibitors like Crizotinib (see Fig. 1). In the cellular phosphorylation assay levels of phospho-ALK are quantified by Sandwich-ELISA technique.

Substrate phosphorylation as a readout of intracellular kinase activity via ELISA

Freiburg, Germany

More information can be found on our website Cellular Phosphorylation Assay Services.