ASH1L-[BRD]-(GST) Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-Ash1L-[BRD]-GST
Protein
Human ASH1L bromodomain, GST-tagged
Synonyms
ASH-1 like protein; huASH1; KMT2H
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.1 mg/ml (2.4 µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
47,5
Reference Compound ΔTms:
No stabilization was observed in the presence of control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, RVX-208, GSK2801 and PFI3)
Scientific Information
Q9NR48 (http://www.uniprot.org/uniprot/Q9NR48)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
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