BAZ1B-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-BAZ1B-His
Protein
Human BAZ1B bromodomain, His-tagged
Synonyms
hWALp2; WSTF
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.1 mg/ml (5.9 µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
44,5
Reference Compound ΔTms:
No stabilization was observed in the presence of control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, RVX-208, GSK2801 and PFI3)
Scientific Information
Q9UIG0 (http://www.uniprot.org/uniprot/Q9UIG0)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
PDF