BAZ2B-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-BAZ2B-H
Protein
Human BAZ2B bromodomain, his-tagged
Synonyms
Bromodomain adjacent to zinc finger domain protein 2B; hWALp4; KIAA1476
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.1 mg/ml (6.2 µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
53
Reference Compound ΔTms:
GSK2801 – 3.5 (red)No stabilization was observed in the presence of additional control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, RVX-208 and PFI3)
Scientific Information
Q9UIF8 (http://www.uniprot.org/uniprot/Q9UIF8)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
PDF