CREBBP-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-CREBBP-GST
Protein
Human CREBBP bromodomain, GST-tagged
Synonyms
CREB-binding protein, CBP
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.1 mg/ml (2.44 µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
39
Reference Compound ΔTms:
CBP112 – 8.5 (Blue)SGC-CBP30 – 9.5 (Red)No stabilization was observed in the presence of other control compounds (JQ1, RVX-208, GSK2801 and PFI3)
Scientific Information
Q92793 (http://www.uniprot.org/uniprot/Q92793)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
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