TAF1L-Tndm-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-TAF1L-T-GST
Protein
Human TAF1L-T bromodomain, GST-tagged
Synonyms
TAF(II)210, TBP-associated factor 1-like, TBP-associated factor 210 kDa, Transcription initiation factor TFIID 210 kDa subunit
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.1 mg/ml (1.7 µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
40,5
Reference Compound ΔTms:
GSK2801 – 1.5 (Yellow)No stabilization was observed in the presence of other control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, RVX-208, and PFI3)
Scientific Information
Q8IZX4 (http://www.uniprot.org/uniprot/Q8IZX4)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
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