Cell-based epigenetic assay for the detection of the relative activity of histone deacetylase (HDAC) class IIa enzymes from cells
Target Overview
Cell Line
THP-1 human acute monocytic leukemia cell line from ATCC
Assay Properties
Assay Format
HDAC-Glo
Assay Protocol
THP-1cells were seeded into the wells of 384-well plate. The cells were treated with reference compound TMP269 (starting at 100 µM, 10-dose with 3-fold dilution) for 1 hour. HDAC Class IIa activity was measured with HDAC-Glo Class IIa Assay on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose-response equation.
Readout
Measurement for deacetylation of the peptide substrate via luminescence
Reference Compound IC50s
| Compound | IC50 (µM) |
|---|---|
| TMP269 | 0.8095 |
Screening location
Malvern, PA, USA
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