CSNK1G2 NanoBRET Kinase Assay

CSNK1G2 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.

Target Overview

Target

CSNK1G2

HGNC Symbol

CSNK1G2

Synonyms

CK1g2, CK1-gamma2, CK1gamma2

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-CSNK1G2 Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-10 and then treated with reference compound CTx-0294885 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.

Readout

Binding of compound to target kinase

Reference Compound IC50s

Compounds	IC50 (M)
CTx-0294885	6.255e-007

Screening Location

Malvern, USA

Further Information

More information can be found on our website NanoBRET Target Engagement Intracellular Kinase Assay Services

Reference compound IC50 for CSNK1G2

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