MAP4K2 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
Target Overview
Target
MAP4K2
HGNC Symbol
MAP4K2
Synonyms
BL44; GCK; RAB8IP
Cell Line
HEK293 human epithelial cell line from ATCC
Assay Properties
Assay Format
384-well plate format
Assay Protocol
HEK293 cells transiently expressing MAP4K2-NanoLuc fusion vector were seeded into the wells of 384-well plates. The cells were pre-treated with the tracer 05 and then treated with reference compound CTx-0294885 for 1 hour. NanoBRET signal was measured on an Envision 2104 Multilabel Reader. IC50 (EC50) value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose-response equation.
Readout
Binding of compound to target kinase
Reference Compound IC50s
| Compounds | IC50 (M) |
|---|---|
| CTx-0294885 | 8.446e-007 |
Screening Location
Malvern, USA
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