CDK19/cyclin C NanoBRET Kinase Assay

CDK19/cyclin C NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
Target Overview

Target

CDK19+Cyclin C

HGNC Symbol

CDK19/CCNC

Synonyms

CDC2L6, CDK11

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently co-expressing NanoLuc- CDK19 fusion vector and Cyclin C Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-8 and then treated with reference compound AST-487 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.

Readout

Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
AST-487 1.123e-008

Screening Location

Malvern, USA

Further information

More information can be found on our website NanoBRET Target Engagement Intracellular Kinase Assay Services.

Reference Compound IC50 for CDK19 + Cyclin C

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