HDAC5 Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
HDAC5
Enzyme
Human HDAC5
Synonyms
Histone Deacetylase 5, Class IIa
Substrates
Fluorogenic HDAC Class2a Substrate (Boc-Lys(trifluoroacetyl)-AMC), 50 μM
Assay Properties
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Trichostatin A | 2,800 |
| SAHA | 27,200 |
| TMP269 | 210 |
Scientific Information
Q9UQL6 (http://www.uniprot.org/uniprot/Q9UQL6)
Screening Location
Malvern, USA
Reference Compound IC50 for HDAC5
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