SIRT1 Sirtuin Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
SIRT1
Enzyme
Human SIRT1 (NAD+-dependent enzyme)
Synonyms
Sirtuin 1, protein-lysyl-deacetylase, Class III HDAC
Assay Properties
Readout
Enzymatic activity
Substrates
Fluorogenic peptide from p53 residues 379-382 (RHKKAc), 50 μM
NAD+, 500 μM
Reference Compound IC50s
| Compounds | IC50 (μM) |
|---|---|
| Suramin sodium | 2.42 |
| Resveratrol | Activator |
Scientific Information
Q96EB6 (http://www.uniprot.org/uniprot/Q96EB6)
Screening Location
Malvern, USA
Reference Compound IC50 for SIRT1
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