FLT3 (K663Q) NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
Target Overview
Target
FLT3 (K663Q)
HGNC Symbol
FLT3 (K663Q)
Synonyms
FLK2; STK1; CD135
Cell Line
HEK293 human epithelial cell line from ATCC
Assay Properties
Assay Format
384-well plate format using Promega’s NanoBRETTM TE Intracellular Kinase Assay
Assay Protocol
HEK293 cells transiently expressing NanoLuc®- FLT3 (K663Q) Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRETTM Tracer K-10 and then treated with reference compound Foretinib for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted
Readout
Binding of compound to target kinase
Reference Compound IC50s
Foretinib (IC50 value): 1.323e-009 M
Screening Location
Malvern, USA
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