HRAS NanoBRET Target Engagement Intracellular Ras Assay for screening of compounds binding to the target.
Target Overview
Target
HRAS
HGNC Symbol
HRAS
Synonyms
HRAS1, RASH1
Cell Line
HEK293 human epithelial cell line from ATCC
Assay Properties
Assay Format
384-well plate format using Promega’s NanoBRET TE Intracellular Ras Assay
Assay Protocol
HEK293 cells transiently expressing LgBiT®-HRAS and SmBiT®-HRAS Fusion Vectors were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-1 and then treated with reference compound BI-2852 for 2 hours. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.
Readout
Binding of compound to target Ras
Reference Compound IC50s
| Compounds | IC50 (M) |
|---|---|
| BI-2852 | 8.727e-008 |
Screening Location
Malvern, USA
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