Activity-based ZAK/MLTK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ZAK/MLTK
Enzyme
Human ZAK/MLTK
HGNC
MAP3K20
Synonym
MLTK, mlklak, ZAK, HCCS-4
Scientific Information
Q9NYL2 (http://www.uniprot.org/uniprot/Q9NYL2)
Assay Properties
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for ZAK/MLTK
