BRD4-2-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-BRD4-2-H
Protein
Human BRD4-2 bromodomain, His-tagged
Synonyms
HUNK1
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.1 mg/ml (6.3 µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
50
Reference Compound ΔTms:
JQ1 – 8 (Orange)PFI1 – 3.5 (Pink)BET151 – 5.5 (Blue)RVX-208 – 2.5 (Purple)No stabilization was observed in the presence of other control compounds (CBP112, SGC-CBP30, GSK2801 and PFI3)
Scientific Information
O60885 (http://www.uniprot.org/uniprot/O60885)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
