CREBBP-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-CREBBP-H
Protein
Human CREBBP bromodomain, His-tagged
Synonyms
CREB-binding protein, CBP
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.1 mg/ml (6.0 M)
Concentration of compound in assay
25 M
Apo form Tm (°C)
42,5
Reference Compound ΔTms:
SGC-CBP30 – 9.5 (Red)No stabilization was observed in the presence of other control compounds (JQ1, PFI1, RVX-208, GSK2801 and PFI3)
Scientific Information
Q92793 (http://www.uniprot.org/uniprot/Q92793)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
