SP100-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-SP100-H
Protein
Human SP100 bromodomain, His-tagged
Synonyms
Nuclear dot-associated Sp100 protein
Assay Properties
Readout
Thermal Stability Assay (TSA)
Concentration of protein in assay
0.125 mg/ml (5.2 µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
51,5
Reference Compound ΔTms:
No stabilization was observed in the presence of control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, RVX-208, GSK2801 and PFI3)
Scientific Information
P23497 (http://www.uniprot.org/uniprot/P23497)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
