TGFBR2 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
TGFBR2
TGFBR2
HNPCC6, MFS2, RIIC, TGFbeta-RII, TBR-II, TRBII
HEK293 human epithelial cell line from ATCC
384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay
HEK293 cells transiently expressing NanoLuc-TGFBR2 Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-11 and then treated with reference compound APY-69 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.
Binding of compound to target kinase
| Compound | IC50 (M) |
|---|---|
| APY-69 | 1.616e-007 |
Malvern, PA, USA
More information can be found on our website NanoBRET Target Engagement Intracellular Kinase Assay Services.
