PP2C-gamma (PPM1G) Phosphatase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
PP2C-gamma
Enzyme
Human PP2C-gamma
Synonyms
PPM1G, PPM1C, Protein phosphatase 1G, Protein phosphatase 1C
Scientific Information
O15355 (https://www.uniprot.org/uniprotkb/O15355)
Assay Properties
Substrate
Fluorogenic substrate DiFMUP, 50 μM
Reaction
[protein]-serine/threonine phosphate + H₂O = [protein]-serine/threonine + phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (mM) |
|---|---|
| PTP1B Inhibitor | 24.5 |
| NSC-95397 | 32 |
| Sanguinarine chloride | >100 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Phosphatase Assay Services for Drug Discovery
Reference compound inhibition of PP2C-gamma (PPM1G)
