PTEN Phosphatase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
PTEN
Enzyme
Human PTEN
Synonyms
MMAC1, TEP1
Scientific Information
P60484 (https://www.uniprot.org/uniprotkb/P60484)
Assay Properties
Substrate
Fluorogenic substrate DiFMUP, 10 μM
Reaction
1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + phosphate
Readout
Enzymatic activity assay using DiF4MU fluorescence readout
Reference Compound IC50s
| Compound | IC50 (mM) |
|---|---|
| PTP1B Inhibitor | 3.8 |
| VO-Ohpic trihydrate | 0.31 |
| SF1670 | 0.23 |
| bpV(phen) trihydrate | 0.009 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Phosphatase Assay Services for Drug Discovery
Reference compound inhibition of PTEN
