Activity-based GAK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
GAK
Enzyme
Human GAK
HGNC
GAK
Synonym
Cyclin-G-associated kinase
Scientific Information
O14976 (http://www.uniprot.org/uniprot/O14976)
Assay Properties
Substrates
Histone H3 Protein, 20 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 136 |
| Ro 31-8220 | 17,000 |
| GW5074 | 204 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for GAK
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