Activity-based RET (V804M/G810S) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
RET (V804M/G810S)
Enzyme
Human RET (V804M/G810S)
HGNC
RET (V804M/G810S)
Synonym
Proto-oncogene tyrosine-protein kinase receptor ret, C-ret
Scientific Information
P07949 (http://www.expasy.org/uniprot/P07949)
Assay Properties
Substrates
Peptide substrate, [RRRRRRRRRRRVYSTDYYRLFNPS], 20 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 17 |
| PP2 | >100,000 |
| AG1478 | >100,000 |
| EGFR/ErbB Inhibitor | >100,000 |
| D-64406 | 2,700 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 RET (V804M/G810S)
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