RET (G810S/M918T) Kinase Assay Service

Activity-based RET (G810S/M918T) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

RET (G810S/M918T)

Enzyme

Human RET (G810S/M918T)

HGNC

RET (G810S/M918T)

Synonym

Proto-oncogene tyrosine-protein kinase receptor ret, C-ret

Scientific Information

P07949 (http://www.expasy.org/uniprot/P07949)

Assay Properties
Substrates

Peptide substrate, [RRRRRRRRRRRVYSTDYYRLFNPS], 20 µM
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 194
PP2 70
AG1478 1,500
EGFR/ErbB Inhibitor >100,000
D-64406 1,800
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 RET (G810S/M918T)

Reference compound IC50 RET (G810S/M918T)