NanoBRET TE PARP12 Assay Service

NanoBRET Target Engagement Intracellular PARP12 Assay, Competitive binding assay format.

Target Overview

Target#

PARP12

HGNC Symbol

PARP12

Synonym

FLJ22693, PARP-12, ZC3H1, ARTD12

Assay Properties

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular PARP12 Assay

Assay Protocol

HEK293 cells transiently expressing PARP12-NanoLuc Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with PARP Tracer-01 and then treated with reference compound Olaparib for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.

Readout

Binding of compound to target

Reference Compound IC50s

Compounds	IC50 (M)
Olaparib	3.233e-006

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Poly (ADP-ribose) Polymerase (PARP) Assay Services

Reference compound IC50 for PARP12

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