CDK4 + Cyclin D3 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
CDK4 + Cyclin D3
CDK4/CCND3 complex
CMM3; PSK-J3
HEK293 human epithelial cell line from ATCC
384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay
HEK293 cells transiently expressing NanoLuc-CDK4 Fusion Vector and Cyclin D3 were seeded into the wells of 384-well plates. The cells were pre-treated with Tracer K-7 and then treated with reference compound Palbociclib for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.
Binding of compound to target kinase
Compounds | IC50 (M) |
---|---|
Palbociclib | 4.065e-009 |
Malvern, PA, USA
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Reference compound IC50 for CDK4 + Cyclin D3