USP9X deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
USP9X
Enzyme
Human USP9X CD (1554aa-1995aa)
Synonym
DFFRX, FAM, USP9
Scientific Information
Q93008 (https://www.uniprot.org/uniprotkb/Q93008)
Assay Properties
Substrate
100 nM Ubiquitin Rhodamine 110 Protein
Reaction
Ubiquitin Rhodamine 110 = Ubiquitin + Rhodamine 110
Rhodamine 110 fluorescence, kinetic measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| FT709 | 0.11 |
| Wu5 | 14.6 |
| UCH-L1 Inhibitor 2 | 5.5 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Deubiquitination Assays (DUB assays)
Reference Compound IC50 for USP9X
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