USP14 + 26S Proteasome (VS Treated) deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
USP14+Proteasome
Enzyme
Human USP14 (1aa-494aa)
Synonym
TGT
Scientific Information
P54578 (https://www.uniprot.org/uniprotkb/P54578)
Assay Properties
Substrate
100 nM Ubiquitin Rhodamine 110 Protein + 1nM VS Treated 26S Proteasome
Reaction
Ubiquitin Rhodamine 110 = Ubiquitin + Rhodamine 110
Rhodamine 110 fluorescence, kinetic measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| IU1 | 3.3 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Deubiquitination Assays (DUB assays)
Reference Compound IC50 for USP14 + VS Treated 26S Proteasome
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