CYP4F3B for compound screening and profiling assay using luminescent based method to measure cytochrome P450 enzyme activity.
Target Overview
CAT#
CYP4F3B
Enzyme
Human CYP4F3B
Synonym
LTB4H, 20-hydroxyeicosatetraenoic acid synthase, CYPIVF3
Scientific Information
Q08477 (https://www.uniprot.org/uniprotkb/Q08477)
Assay Properties
Substrate
P450-Glo™ proluciferin Substrate
Reaction
RH + O₂ + NADPH + H⁺ = ROH + H₂O + oxidized [NADPH—hemoprotein reductase]
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (nM) |
|---|---|
| TP0472993 | 15.5 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website CYP450 Inhibition Assay Services.
Reference compound IC50 for CYP4F3B
