Activity-based KIF5B Kinesin ATPase assay for compound screening and profiling via ADP-Glo™.
Target Overview
CAT#
KIF5B
Enzyme
Human KIF5B (1-379aa)
Family
Kinesin-1
Synonym
Kinesin-1 heavy chain, Conventional kinesin heavy chain, KNS, KNS1
Scientific Information
P33176 (http://www.uniprot.org/uniprot/P33176)
Assay Properties
Substrates
Tubulin Microtubules, 0.1 mg/ml
ATP, 10 µM
ATP, 10 µM
Reaction
ATP = ADP + Pi
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| Paprotrain | >100 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinesin ATPase Assay Services
Reference compound inhibition of KIF5B
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