Activity-based KIFC1 Kinesin ATPase assay for compound screening and profiling via ADP-Glo™.
Target Overview
CAT#
KIFC1
Enzyme
Human KIFC1 (307-663aa)
Family
Kinesin-14
Synonym
HSET, KNSL2
Scientific Information
Q9BW19 (http://www.uniprot.org/uniprot/Q9BW19)
Assay Properties
Substrates
Tubulin Microtubules, 0.1 mg/ml
ATP, 10 µM
ATP, 10 µM
Reaction
ATP = ADP + Pi
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| BTB-1 | >100 |
| Sovilnesib (AMG 650) | 36 |
| KIF18A-IN-1 | >100 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinesin ATPase Assay Services
Reference compound inhibition of KIFC1
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