Activity-based GAK kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
GAK
Enzyme
Human GAK, wt
HGNC
GAK
Manning ID
GAK
Synonym
auxilin2, DNAJC26, MGC99654
UniProt
Kinase Type
Protein kinase
Kinase Group
Other
Assay Properties
Assay Format
33PanQinaseTM
Substrates
ERK2 K54R, ATP
Reaction
Substrate + 33P-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1600 |
| Sorafenib | 2600 |
| Sunitinib | >10000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | GAK |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – GAK (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1974-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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