20s proteasome assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
20S Proteasome – Chymotrypsin like
Enzyme
Human 20S proteasome purified from human erythrocytes
Substrate
Fluorogenic peptide [Suc-LLVY -AMC], 10 µM
and Activator PA28
Assay Properties
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
| Ixazomib (MLN2238) | 10.4 |
| Carfilzomib (PR-171) | 2.7 |
| Oprozomib (ONX 0912) | 450 |
| PI-1840 | 92 |
Screening Location
Malvern, PA, USA
Further information
More information can be found on our website Ubiquitin-Proteasome Pathway Assay Services.
Reference Compound IC50 for 20S Proteasome
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