KDM4D Demethylase Assay for compound screening and profiling via AlphaLISA-based quantification of enzyme activity.
Target Overview
CAT#
KDM4D
Enzyme
Human KDM4D
Synonym
Lysine-specific demethylase 4D, JmjC domain-containing histone demethylation protein, 3D Jumonji domain-containing protein 2D, JMJD2D
Substrates
B- H3(1-21)-K9Me3, α-KG
Assay Properties
Reaction
H3(1-21)-K9Me3 + α-KG + O₂ = H3(1-21)-K9Me2 + succinate + CO₂ + formaldehyde
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| 2,4-PDCA | 331 |
Scientific Information
Q6B0I6 (http://www.uniprot.org/uniprot/Q6B0I6)
Screening Location
Malvern, USA
Reference compound inhibition of KDM4D
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