KDM5A Demethylase Assay for compound screening and profiling via HTRF-based quantification of enzyme activity.
Target Overview
CAT#
KDM5A
Enzyme
Human KDM5A
Synonym
Lysine-specific demethylase 5A, JARID1A
Substrates
B-H3(1-21)-K4Me3, α-KG
Assay Properties
Reaction
H3(1-21)-K4Me3 + α-KG + O₂ = H3(1-21)-K4Me2 + succinate + CO₂ + formaldehyde
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| 2,4-PDCA | 159 |
Scientific Information
P29375 (http://www.uniprot.org/uniprot/P29375)
Screening Location
Malvern, USA
Reference compound inhibition of KDM5A
PDF