5-hydroxytryptamine (Serotonin) receptor 5-HT2A Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
5-HT2A Binding
GPCR Target
Human 5-HT2A
Source
Membrane preparation from CHO-K1 cells stably transfected with human 5-HT2A receptor.
Scientific Information
HTR2A (https://www.uniprot.org/uniprotkb/E1JJ46/entry)
Assay Properties
Protocol
Radioligand filter binding to 5HT-2A membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter
Readout
CPM representing 3H-disintegration 5-HT2A detected using MicroScint-20
Reference Compound IC50
| Compound IC50 (nM) | Radioligand (nM) |
|---|---|
| Ketanserin (1.1) | LSD (2) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound binding data for 5-HT2A
PDF