CDK8+Cyclin C NanoBRET Kinase Assay

CDK8+Cyclin C NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
Target Overview

Target

CDK8+Cyclin C

HGNC Symbol

CDK8/CCNC complex

Synonyms

K35

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently co-expressing NanoLuc-CDK8 fusion vector and Cyclin C Vector were seeded into the wells of 384-well plates. The cells were pretreated with the NanoBRET Tracer K-8 and then treated with reference compound Staurosporine for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.

Readout

Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
Staurosporine 7.643e-008

Screening Location

Malvern, USA

Reference compound IC50 for CDK8+Cyclin C