Caspase 8 Protease Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
Caspase 8
Enzyme
Human Caspase 8
Synonym
Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, CASP8, MCH5
Substrates
Fluorogenic peptide Ac-LEHD-AMC, 5 µM
Assay Properties
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IETD-CHO | 2.23 |
Scientific Information
Q14790 (https://www.uniprot.org/uniprotkb/Q14790/entry)
Screening Location
Malvern, PA, USA
Reference compound IC50 for Caspase 8
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