FLT3 NanoBRET Kinase Assay

FLT3 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.

Target Overview

Target

FLT3

HGNC Symbol

FLT3

Synonyms

FLK2; STK1; CD135

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRETTM TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc®- FLT3 Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRETTM Tracer K-5 and then treated with reference compound CTx-0294885 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.

Readout

Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
CTx-0294885 5.774e-008

Screening Location

Malvern, USA

Reference Compound IC50 for FLT3

Reference Compound IC50 for FLT3

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