KIT (V559D,T670I) NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
KIT (V559D,T670I)
KIT (V559D/T670I)
C-Kit, PBT, CD117, SCFR
HEK293 human epithelial cell line from ATCC
384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay
HEK293 cells transiently expressing NanoLuc-KIT(V559D,T670I) Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-8 and then treated with reference compound CEP-701 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.
Binding of compound to target kinase
Compounds | IC50 (M) |
---|---|
CEP-701 | 3.290e-007 |
Malvern, USA
![Reference compound IC50 for KIT (V559D,T670I)](https://www.reactionbiology.com/sites/default/files/styles/datasheet_figure/public/2021-04/KIT_%28V559D%2CT670I%29_nano.png?itok=NcHvQg7u)