MTH1 Replication-related Assay for compound screening and profiling via quantification of enzyme activity using the PPiLightTM Inorganic Pyrophosphate Assay.
Target Overview
CAT#
MTH1
Enzyme
Human MTH1
Synonyms
NUDT1, 7,8-dihydro-8-oxoguanine triphosphatase, Nucleoside diphosphate-linked moiety X motif 1
Substrate
15 µM 8-Oxo-2’-dGTP
Assay Properties
Reaction
8-oxo-dGTP + H2O = 8-oxo-dGMP + diphosphate
Readout
Enzymatic activity via PPiLightTM Inorganic Pyrophosphate Assay (Lonza)
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| S-Crizotinib | 428 |
| SCH51344 | 894 |
Scientific Information
Reference compound IC50 for MTH1
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