PIK3CD/PIK3R1 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
PIK3CD/PIK3R1
PIK3CD/PIK3R1
p110D, P110DELTA, PI3K, PI3K-delta, p110-delta
HEK293 human epithelial cell line from ATCC
384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay
HEK293 cells transiently co-expressing NanoLuc-PIK3CD Fusion Vector and PIK3R1 Expression Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-3 and then treated with reference compound PI-103 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.
Binding of compound to target kinase
| Compounds | IC50 (M) |
|---|---|
| PI-103 | 3.197e-008 |
Malvern, PA, USA
Reference compound IC50 for PIK3CD/PIK3R1
