Activity-based ABL1 G250E kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
ABL1 G250E
Enzyme
Human ABL1 G250E, mutant
HGNC
ABL1 (G250E)
Manning ID
ABL1
Synonym
ABL1, ABL, JTK7, p150, c-ABL, v-abl, ABL1 G250E, ABL1 (G250E)
UniProt
P00519 (ABL1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Poly(Ala,Glu,Lys,Tyr)6:2:5:1, ATP
Reaction
Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + 33P-ATP = 33P-Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1167 |
| Sorafenib | 10000 |
| Sunitinb | 27000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | ABL1 G250E |
| 345 wt Profiler | No |
| 345 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | IC50 ABL1 Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – ABL1 G250E (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0925-0000-1 |
| Web Shop | Reaction Biology Online Shop |
PDF