Activity-based ABL1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ABL1
Enzyme
Human ABL1
HGNC
ABL1
Synonym
ABL, JTK7, p150, c-ABL, v-abl
Substrates
Peptide substrate, [EAIYAAPFAKKK], 20 µM
ATP, 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
P00519 (http://www.uniprot.org/uniprot/P00519)
Screening Location
Malvern, USA
Reference compound IC50 for ABL1
